Systems neuroscience

Wagih H. Marcus

Inokuchi Laboratory · Department of Biochemistry · University of Toyama, Japan

  • Memory integration
  • Transitive inference
  • Sleep and offline processing
  • Prefrontal cortex
  • Neuropixels 2.0
Portrait of Wagih H. Marcus at the CNS 2026 meeting
CNS 2026Fuzhou, China
1,203 mPFC neurons · day 19−5+5Odor A
Population trajectory · PC1–3AE test
Recorded data from the first novel-pair test (day 19), six sleep-control mice, Neuropixels 2.0: baseline-normalized activity of 1,203 medial prefrontal neurons on completed trials (sorted by response during odor, three neurons per row), and the population trajectory of correct trials on PC1–PC3 for the AE anchor pair and the BD inference pair.

Research

Research experience

Toyama 2023 – present
Cairo 2022 – 2023

2023 – present

Systems Neuroscience

Inokuchi Laboratory · University of Toyama, Japan

Memory integration, transitive inference and offline prefrontal processing

  • Developed a new head-fixed olfactory paradigm for transitive inference, in which mice learn overlapping odor pairs and are then tested on novel relational choices (first-author manuscript submitted to Molecular Brain).
  • Used post-session chemogenetic inhibition of the medial prefrontal cortex and sleep deprivation to test how offline processing after learning contributes to subsequent inference.
  • Recorded prefrontal populations with Neuropixels 2.0 during behavior and subsequent sleep, and analyzed task-related representations across learning and inference.
  1. 1Learn adjacent pairs
  2. 2Offline, during sleep
  3. 3Infer a new pair
Transitive inference, schematically Five odors A to E. Adjacent pairs are learned: A over B, B over C, C over D and D over E. After an offline period, the new pair B versus D is inferred: B over D. sleep A > B B > C C > D D > E A B C D E B > Dnever trained together
Schematic of a transitive inference task. Odors A–E follow a hidden order. Training uses only adjacent pairs, so choosing B over D is an inference.
2022 – 2023

Neuropharmacology

Faculty of Pharmacy · Cairo University, Egypt

Natural coumarins in mouse models of schizophrenia-like behavior, mood and cognition

  • Investigated natural compounds in preclinical mouse models of cognition and of depression-, anxiety- and schizophrenia-related behavior.
  • Collaboration with the Medical University of Lublin, Poland; published in European Journal of Pharmacology (2025, co-first author) and Acta Poloniae Pharmaceutica – Drug Research (2025, first author).
  1. 1Plant coumarins
  2. 2Umbelliferone
  3. 3Scoparone
Natural coumarins in mouse models, schematically Coumarins are plant-made compounds. Umbelliferone, or 7-hydroxycoumarin, was assessed for cognitive, depression- and anxiety-related effects in mice. Scoparone, or 6,7-dimethoxycoumarin, was tested against schizophrenia-like behavior induced by the NMDA receptor antagonist MK-801, with a focus on the SIRT1, PGC-1 alpha and TFAM pathway that drives mitochondrial biogenesis. Umbelliferae, the carrot family O O HO H3CO H3CO Coumarinthe plant-made scaffold Umbelliferone7-hydroxycoumarin Scoparone6,7-dimethoxycoumarin Cognition Depression-related Anxiety-related behavior in mice MK-801 NMDA antagonist Schizophrenia-like behavior mechanism SIRT1 PGC-1α TFAM Mitochondrial biogenesis
Schematic of the natural-product work. Umbelliferone (7-hydroxycoumarin) was profiled for cognitive, depression- and anxiety-related effects; scoparone (6,7-dimethoxycoumarin) was tested against MK-801-induced schizophrenia-like behavior, with a focus on the SIRT1/PGC-1α/TFAM pathway.

Education

From Cairo to Toyama

9,317 km apart
2015 – 2027

Egypt30.04° N · 31.24° E

2015 – 2020

Cairo University

Faculty of Pharmacy

Bachelor of Pharmacy and Pharmaceutical Sciences

  • Summa cum laude
  • Valedictorian
  • Ranked first in a class of over 1,000
  • First in every year of study

2021 – 2022Graduate coursework in Pharmacology and Toxicology · GPA 4.0/4.0

Japan36.70° N · 137.21° E

2023 – 2027

University of Toyama

Inokuchi Laboratory · Department of Biochemistry

PhD in Cognitive and Emotional Neuroscience

  • Supervisor: Prof. Kaoru Inokuchi
  • Uehara Memorial Foundation grant
  • Otsuka Toshimi scholarship

2023 – 2027Graduate Teaching and Research Assistant, Faculty of Medicine

Journey

Seven cities, four countries

2 universities · 5 meetings
2015 – 2026

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Overview

Cairo to Fuzhou, 2015 – 2026

Two universities and five scientific meetings. Select a place to fly there, or play the journey in order.

Publications

Published work

5 peer-reviewed · 1 submitted
4 as first or co-first author

  1. Submitted

    A novel head-fixed olfactory paradigm for transitive inference in mice

    Marcus WH, Nomoto M, Inokuchi K.

    Molecular Brain · submitted 2026

  2. 2026

    A holistic guide to effective prevention and treatment for kidney stones: a systematic review exploring antiurolithiasis approaches

    Allam AT, El-Dessouki AM, El-Shiekh RA, Abou-Hussein D, Mahmoud MA, Marcus WH, Ruby HA.

    Naunyn-Schmiedeberg’s Archives of Pharmacology · 2026;399:3235–3284

  3. 2025

    Assessing cognitive, depression-, and anxiety-related effects of umbelliferone in mice

    Marcus WH, Ruby HA, Mousa MO, El Sayed NS, Budzynska B, Biała G, Skalicka-Woźniak K.

    Acta Poloniae Pharmaceutica – Drug Research · 2025;82(6):983–995 · published online May 2026

  4. Modulatory effects of bioactive natural compounds on pruritic pathways: mechanistic basis and therapeutic prospects

    Marcus WH, Ruby HA, Awwad AE, Mahrous MH, Abouelwafa E, Badawy MAS, El-Shiekh RA, Zaki ES, Mahmoud MA.

    Inflammopharmacology · 2025;33:7079–7106

  5. Repositioning of a nutraceutical, scoparone, in the management of MK-801-induced schizophrenia-like behavior in mice: an insight into SIRT1/PGC-1α/TFAM pathway

    Rabie MA*, Marcus WH*, Sadek MA*, Ruby HA, Emam KAF, Budzynska B, Skalicka-Woźniak K, Sayed RH, Ahmed LA, El Sayed NS.

    European Journal of Pharmacology · 2025;1004:177981

  6. In vitro and in vivo studies of natural anti-acne treatments from pomegranate pericarp extract and essential oils of bitter orange, sweet marjoram, and tea tree

    Abozeid DO, Soliman FM, Fawzy GA, Abdeltawab NF, Al-mahallawi AM, Emam KAF, Marcus WH, Issa MY.

    Future Journal of Pharmaceutical Sciences · 2025;11:110

* Equal contribution. Full list on Google Scholar.

Honors

Fellowships and honors

¥5.6 million in scholarships and grants
US$800 travel award

¥2,000,000scholarship

Otsuka Toshimi Scholarship Foundation Scholarship

2026 – 2027

¥3,600,000graduate grant

Uehara Memorial Foundation Graduate Student Grant

2024 – 2025

’23’24’25

×3consecutive years

Outstanding Poster Presentation Awards

University of Toyama

2023 · 2024 · 2025

1stof over 1,000

Valedictorian and Excellence with Honors

Faculty of Pharmacy, Cairo University

2020

Experience

Appointments and teaching

2 universities
2016 – 2027

Academic appointments

  1. 2023 – 2027

    Graduate Teaching and Research Assistant

    Department of Biochemistry, Faculty of Medicine, University of Toyama, Japan

  2. 2022 – 2023

    Teaching and Research Assistant

    Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Egypt

Teaching and mentoring

2023 – present

University of Toyama

Medical English and biochemistry for medical students; experimental training and laboratory journal clubs.

2022 – 2023

Cairo University

Pharmacology I–III, Toxicology and Bioassay for undergraduate and graduate students.

Undergraduate internships

Industry R&D
Hikma Pharmaceuticals (2019); EVA Pharma (2018)
Research
Pharmaceutical organic chemistry and microbiology/immunology, Cairo University (2018)
Pharmacy
Community pharmacy, Hany Pharmacy (2016 and 2017)

Expertise

Technical expertise

From the bench
to the population code

Neuropixels 2.0 shank
schematic
Recording
Neuropixels 2.0 recordings of prefrontal populations during behavior and subsequent sleep
Behavior
Head-fixed olfactory operant conditioning; transitive inference paradigm design; mouse models of cognition and of depression-, anxiety- and schizophrenia-related behavior
Surgery
Stereotaxic surgery; viral vector delivery
Analysis
Task-related population representations across learning and inference; code and materials on GitHub
Pharmacology
Preclinical evaluation of natural compounds; systematic and mechanistic literature reviews

Beyond the lab

Beyond the laboratory

Games · reading · karaoke
films · collecting · travel

Games · play a round

Chess, Monopoly, Yatzy and Nintendo

Setting up the board…

You play White against Stockfish, one of the strongest chess engines; pick your colour before the first move. Tap a piece, then one of the marked squares. Every rule applies: castling, en passant, promotion, check, checkmate and all the draws.

No moves yet.

Reading · on the shelf

History, politics and philosophy

  • Philosophy
  • Literature
  • Mythology
  • History
  • Politics

Collecting

Tomica die-cast scale models of Japanese Red Cross vehicles

Watching

Ten favourite films and ten favourite series

The projector runs the films; switch to series and the television takes over.

Karaoke

A microphone and eight favourite artists

References

References

3 referees

PhD supervisor

Prof. Kaoru Inokuchi

Department of Biochemistry, University of Toyama, Japan

Co-author

Assoc. Prof. Masanori Nomoto

Department of Biochemistry, University of Toyama, Japan

Cairo University

Prof. Nesrine Salah El-Din El-Sayed

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Egypt

Contact details for referees are available on request.

Contact

Write to me

Questions about the work, collaborations, or a referee's contact details: send a note and I will reply by email.

ToWagih H. MarcusInokuchi Laboratory, University of Toyama

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